If the microscope sample uses styrene resin as the embedding agent, it is very convenient to perform the scanning electron microscope observation by cutting. Because styrene is polymerized, propylene oxide and isovaleric acid can be easily dissolved. The styrene can be removed by replacing the solvent several times. Therefore, when making TEM specimens, several embedding capsules are made. After the sample is to be polymerized, the microscope is cut according to the above method, and the McAudi microscope will place the cut sample in propylene oxide, and after about 2 hours, it can be replaced by isocyanuric acid and the critical point is dry. radiopaque adhesive tape,Radiopaque Tape,Medical Tape Anesthesia Medical Co., Ltd. , https://www.trustfulmedical.com
In addition to the resin cutting method, there are organic solvent (alcohol, isovaleric acid, etc.) cutting method, microscope water-soluble embedding agent (dimethyl hydrazine, glycerin, etc.) cutting method and Others (freezing, slave, etc.) The cutting method is easy to operate. Take the alcohol cutting method as an example: the sample is fixed and dehydrated with alcohol. When the dehydration is complete, the microscope takes the sample into the capsule containing the anhydrous ether (avoiding bubbles). The microscope placed the capsule in a low-temperature pack to cure the drunk. The sample was cut. The cut sample immediately enters the water-free drunk at room temperature, and after being melted, it is transferred to isoamyl acetate and subjected to a critical point of dry operation.
It should be pointed out that the specimen prepared by the cutting method needs to be observed with a high-resolution microscope (such as a field emission electron gun scanning electron microscope). The general scanning electron microscope has a lower resolution than the transmission electron microscope. If the microscope and the microscopic method are used to observe the tissue and the cells. The internal structure, in contrast to the transmission electron microscopy image, must be scanned with a field emission electron gun. Due to the strong stereoscopic effect of the microscope, the cut sample shows the spatial distribution of the Golgi flat sac and the large vesicles and vesicles under the microscope. The mitochondria wax is like a partition. Stretching; the nucleus is attached to the "plate" of the endoplasmic reticulum like a small mushroom. Despite this, some scholars believe that polarized light microscopy does not provide new information on cell structure. Therefore, the method is not widely used.
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How to use the microscope to observe with the cutting method