Frac hoses, were applied widely in oil and gas field, also can be used for fire brigade to transfer water with long-distance.
According to the different material, Frac Hose include NBR fire hose, TPU frac hose.
According to different diameter, frac hose include small diameter fire hose and big diameter frac hose.
Frac hoses were produced through-the-weave, easy cleaning and no drying required.
Designed for long service life. Tough and durable with exceptional resistance to abrasion
And cutting for use on wide variety of environment conditions.
Protected against mechanical damages.
Very low elongation (compared with vulcanized process) under pressure, prevents hose from [snaking".
High resistance to heat, fuels, chemicals, UV, ozone, weathering,hydrolysis,and microbiological attack.
Tube & cover: thermoplastic, polyurethane (TPU)
Reinforcement: High tenacity polyester filament, circular weaving.
The special extrusion through-the-weave process gives a very strong bonding between cover and lining as well as firmly encapsulation the woven polyester.
Temperature range from -50℃ to +70℃.
We can supply frac hoses with size: 8``,10``,12`` and 16``.
Frac Hose High Pressure Hose,High Pressure Fire Hose,High Pressure Hydraulic Hose,High Pressure Rubber Hose NANTONG SENTIAN FIRE-FIGHTING EQUIPMENT CO.,LTD. , https://www.firehosefactory.com
Rat Bone Molding Protein-4 (BMP-4) ELISA Kit Instructions
Shanghai Xitang Biotechnology Co., Ltd. 021-55229872, 65333639
Rat bone molding protein -4 (BMP-4) ELISA kit
 ( for serum, plasma, cell culture supernatants and biological fluids )
principle
This experiment used double antibody sandwich ABC-ELISA. The anti-rat BMP-4 monoclonal antibody was coated on the plate, the BMP-4 in the standard and the sample was combined with the monoclonal antibody, and biotinylated anti-rat BMP-4 was added to form an immune complex attached to the plate. On the horseradish peroxidase-labeled Streptavidin combined with biotin, the substrate working solution is blue, and finally the stop solution sulfuric acid is added, and the OD value is measured at 450 nm. The BMP-4 concentration is proportional to the OD value, and can be passed. A standard curve was drawn to determine the BMP-4 concentration in the specimen.
Kit composition ( 2-8 ° C preservation)
Coated Wells
96 holes
Enzyme Conjugate
12ml
10× specimen dilution (Sample Buffer)
12ml
20×Wash Buffer
50ml
Standards: 20ng/bottle
2 bottles
Substrate working fluid (TMB Solution)
12ml
Primary antibody working solution (Biotinylated Antibody)
12ml
Stop Solution
12ml
Prepare reagents and collect blood samples
1. Collection of specimens: serum, plasma (EDTA, citrate, heparin anticoagulation), cell culture supernatant, tissue homogenate, etc., as early as possible, stored at 2-8 ° C for 48 hours; longer time must be frozen (-20 °C or -70 °C) Save to avoid repeated freezing and thawing.
2. Standard solution preparation: Add 1 ml of distilled water before use and mix well to prepare a 20 ng/ml solution. Set the standard tube 8 tube, the first tube plus the standard dilution 900ul, the second to the eighth tube to add the sample dilution 500ul. Add 100 ul of 20 ng/ml standard solution to the first tube, mix and aspirate 500 ul with the sampler, and transfer to the second tube. Repeat the dilution as described above, and remove 500 ul from the seventh tube and discard it. The eighth tube is a blank control.
3. The 10× specimen dilution was diluted 1:10 with distilled water (example: 1 ml concentrated dilution + 9 ml distilled water).
4. Washing solution: diluted 1:20 with distilled water (example: 1 ml concentrated washing solution added to 19 ml of distilled water)
Test procedure
1. Loading: Add 100 ul of standard or sample to be tested in each well. Mix the reaction plate thoroughly and let it stand at 37 °C for 120 minutes.
2. Wash the plate: Wash the plate thoroughly with washing solution 4-6 times, and dry it on the filter paper.
3. Add 100 ul of the first antibody working solution to each well. The reaction plate was thoroughly mixed and placed at 37 ° C for 60 minutes.
4. Wash the board: the same as before.
5. Add 100 ul of enzyme-labeled antibody working solution per well. The reaction plate was placed at 37 ° C for 30 minutes.
6. Wash the board: same as before.
7. Add 100 ul of substrate working solution per well, set 37 The reaction was carried out in the dark at °C for 15 minutes.
8. Add 100 ul of stop solution to each well and mix.
9. Measure the absorbance at 450 nm using a microplate reader within 30 minutes.
Result calculation and judgment
1. All OD values ​​should be subtracted from the blank value before calculation.
2. Take the standard products 2000, 1000, 500, 250, 125, 62.5, 31.2, 0 pg/ml as the abscissa and OD as the ordinate. Draw on the coordinate paper and draw the standard curve.
3. Find the corresponding BMP-4 content on the graph based on the OD value of the sample.
Kit performance
1. Sensitivity: The minimum BMP-4 detection concentration is less than 15pg/ml.
2. Specificity: Recombinant or natural rat BMP-4 can be detected simultaneously. Does not cross-react with other cytokines in rats.
3. Repeatability: The coefficient of variation in the plate and plate is less than 10.2%.
Precautions
1. It is recommended to make double holes for the above standard holes and samples to be tested. The standard curve should be made at the same time for each measurement.
2. The washing process is critical. Insufficient washing will result in an accuracy error and an erroneous rise in the OD value.
3. After the slats are opened, the remaining slats should be sealed again to keep the slats dry .
4. This kit should be stored in a 4 o C refrigerator.
5. This kit is for scientific research only and cannot be used for clinical diagnosis!